phospho antibody microarrays  (Kinexus Bioinformatics Corporation)

Journal: bioRxiv

Article Title: Plasmodium falciparum exploits NUAK1 to establish infection in human erythrocytes

doi: 10.1101/2025.10.30.685469

Figure Lengend Snippet: a , Schematic representation of ex-vivo erythropoiesis protocol to generate isogenic WT and CD44-null cRBC from primary human CD34+ HSPCs, followed by Kinexus phosphoantibody microarrays. b , Western blot of NUAK1 Thr211 phosphorylation in whole cell lysates from WT and CD44-null mature cRBCs (day 21) +/-EBA-175 stimulation. c , Western blot of NUAK1 Thr211 in RBC supernatant fractions from uninfected (uRBC) and P. falciparum -infected RBCs (iRBC). d , Western blot of NUAK1 expression in primary human CD34+ HSPC-derived erythroblasts at indicated dates of ex-vivo differentiation.

Article Snippet: To investigate the CD44-dependent signaling induced by EBA-175, we generated isogenic wild-type (WT) or CD44-null cultured red blood cells (cRBCs) from primary human hematopoietic stem/progenitor cells (HSPCs) and used phospho-antibody microarrays produced by Kinexus ( ).

Techniques: Ex Vivo, Western Blot, Phospho-proteomics, Infection, Expressing, Derivative Assay

Journal: International Journal of Molecular Sciences

Article Title: Senescent Secretome of Blind Mole Rat Spalax Inhibits Malignant Behavior of Human Breast Cancer Cells Triggering Bystander Senescence and Targeting Inflammatory Response

doi: 10.3390/ijms24065132

Figure Lengend Snippet: ( A ) Heatmap representing differences in the levels of expression/phosphorylation of NF-κB—related proteins in MDA-MB-231 cells exposed to Spalax CM (Treated) compared to control; ( B ) Volcano plot representing the log2 FC (treatment/control) versus −log10 p value (cut-off range— p value ≤ 0.05). Dots represent individual proteins.

Article Snippet: An antibody microarray for phospho-NF-κB PNK215 (Fullmoon, Biosystems, Sunnyvale, CA, USA) was performed using protein lysates from MDA-MB-231 cells, either untreated or treated with Spalax CM.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Senescent Secretome of Blind Mole Rat Spalax Inhibits Malignant Behavior of Human Breast Cancer Cells Triggering Bystander Senescence and Targeting Inflammatory Response

doi: 10.3390/ijms24065132

Figure Lengend Snippet: Spalax CM suppresses membrane-bound IL1-α and inhibits phosphorylation of NF-κB and p38 in MDA-MB 231 cells. ( A ) Representative images showing MDA-MB231 untreated and treated with Spalax (Sp) and human (Hum) CMs. A magnified view of the enclosed regions is shown on a low panel, highlighting the differences in IL1-α distribution within the cells between treated with human and Spalax CMs for 24 h and untreated. Treated/untreated cells were stained with IL1α–FITC and NF-κB-p65 antibodies; nuclei were counterstained with DAPI. Arrows indicate the presence of IL1-α on the membrane ( B ) Representative images of flow cytometry showing differences in IL1α surface membrane in MDA-MB-231 untreated, treated with Spalax CM, human CM, with or without recombinant human IL-1α protein. ( C ) Representative western blot analysis demonstrating the phosphorylation of p65 (Ser536), p38 in MDA-MB231 treated with recombinant IL-1α, Spalax CM (SpCM), Spalax CM+IL-1α (SpCM+IL-1α), and human CM (Hu CM). ( D ) Densitometry quantification of western blots. Western blots and densitometries of additional biological repeats (MDA-MB-231 treated with Spalax CMs generated from fibroblasts of different Spalax individuals) are presented in .

Article Snippet: An antibody microarray for phospho-NF-κB PNK215 (Fullmoon, Biosystems, Sunnyvale, CA, USA) was performed using protein lysates from MDA-MB-231 cells, either untreated or treated with Spalax CM.

Techniques: Staining, Flow Cytometry, Recombinant, Western Blot, Generated